Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers

Instability of s male-sterile cytoplasm in maize.

A number of S male-sterile plants from several shrunken-2 inbred lines were crossed initially with an R138-TR inbred line pollinator carrying the nonrestoring genotype for S sterile cytoplasm. One such cross, involving a male-sterile female parent from inbred line M825, produced, unexpectedly, a number of male-fertile F(1) progeny, along with the expected male-sterile off-spring. Pollen records...

Application of specific primers and polymerase chain reaction for identification of ralstonia solanacearum biovar2

Specific Primers For Identification and Detection of Three Major Desert Truffle Species in Iran with Special Reference to Terfezia claveryi

ABSTRACT- Desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. A PCR-based method was developed for the identification of 3 major desert truffles of Iran: Terfezia claveryi, Tirmania pinoyi and Tirmania nivea based on internal transcribed spacers of rDNA. Two specific PCR primers were designed for T. claveryi, 4 for T. pinoyi, and 2 for ...

Identification of Self- incompatibility Alleles in Some Almond Genotypes by Degenerate S-RNase Primers

The almond, Prunus dulcis Miller which belongs to Rosaceae family, is one of the most important commercial and oldest cultivated tree nut crops. Almonds are classified as a ‘nut’ in which the edible seed is the commercial product. Therefore, pollination and fertilization are necessary in almond. The characteristic of cultivated almond to express gametophytic self- incompatibility discourages se...

RAPID IDENTIFICATION OF Vibrio cholera-01 BY COAGGLUTINATION TEST USING MONO-SPECIFIC ANTIBODY

Mono-specific antisera against Vibrio cholera Ogawa NIH-43 and Vibrio cholera Inaba 35-A3 were prepared from rabbit hyperimmune sera by absorbing against a heterologous strain. Using ammonium sulphate precipitation procedure, gamma globulins were purified and concentrated. To visualize antigen-antibody reaction, gamma globulins were conjugated to Staphylococcus aureus cowan-l (NCTC: 8325) ...